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anti scd1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti scd1
    Anti Scd1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+scd1+antibody/SCD1+Rabbit+mAb/pm41935215-265-21-22
    Average 95 stars, based on 224 article reviews
    anti scd1 - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Protein Concentration:

    Article Title: Exercise mitigates flow recirculation and activates metabolic transducer SCD1 to catalyze vascular protective metabolites
    Article Snippet: .. Protein concentration was measured using the Bio-Rad DC assay and 50 μg of protein was loaded for Western blot with anti-SCD1 antibody (Cell Signaling) and anti-β-tubulin (Millipore, Inc.) for loading normalization. ..

    Article Title: Mechano-Genomic Analysis to Identify Flow-Mediated Vascular Transducers: A Multi-Scale Approach
    Article Snippet: .. Protein concentration was measured using the Bio-Rad DC assay and 50 μg of protein was loaded for Western blot with anti-SCD1 antibody (Cell Signaling). .. Parallel blots were performed with anti-β-tubulin (Millipore, Inc.) for loading normalization.

    Western Blot:

    Article Title: Exercise mitigates flow recirculation and activates metabolic transducer SCD1 to catalyze vascular protective metabolites
    Article Snippet: .. Protein concentration was measured using the Bio-Rad DC assay and 50 μg of protein was loaded for Western blot with anti-SCD1 antibody (Cell Signaling) and anti-β-tubulin (Millipore, Inc.) for loading normalization. ..

    Article Title: Mechano-Genomic Analysis to Identify Flow-Mediated Vascular Transducers: A Multi-Scale Approach
    Article Snippet: .. Protein concentration was measured using the Bio-Rad DC assay and 50 μg of protein was loaded for Western blot with anti-SCD1 antibody (Cell Signaling). .. Parallel blots were performed with anti-β-tubulin (Millipore, Inc.) for loading normalization.

    SDS Page:

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 μ g of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or anti-SREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 mg of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or antiSREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Membrane:

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 μ g of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or anti-SREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 mg of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or antiSREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Incubation:

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 μ g of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or anti-SREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Article Title: Stearoyl-CoA desaturase 1 and paracrine diffusible signals have a major role in the promotion of breast cancer cell migration induced by cancer-associated fibroblasts.
    Article Snippet: To evaluate the expression level of the nuclear mature form of SREBP1, nuclear proteins were extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific, Waltham, MA, USA). .. In all 30 mg of total or nuclear proteins were resolved on a 10% SDS-PAGE and transferred onto Immobilon P membrane (Millipore, Bedford, MA, USA) which was incubated with anti-SDF-1 antibody (Abcam, Cambridge, UK, 1 : 1000), anti-SCD1 antibody (clone M38, Cell Signaling Technology, Danvers, MA, USA, 1 : 2000), or antiSREBP1 antibody (clone C20, Santa Cruz Biotechnology, Santa Cruz, CA, 1 : 200). .. The blot was then overlaid with a HRP-labelled secondary antibody (Vector Laboratories, Burlingame, CA, USA; 1 : 3000 dilution).

    Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis
    Article Snippet: Subsequently, proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. After blocking with 5% bovine serum albumin (BSA) in TBST (Tris-buffered saline with Tween 20) for 1 h, the membranes were washed and incubated sequentially with a primary anti-SCD1 antibody (#2794, Cell Signaling Technology, Beverly, MA, USA) and anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies. .. Specific antibody binding was visualized using an enhanced chemiluminescence (ECL) detection reagent (Invitrogen, Carlsbad, CA, USA).

    Article Title: BF175 inhibits endometrial carcinoma through SREBP-regulated metabolic pathways in vitro
    Article Snippet: Then the proteins were separated on SDS-PAGE gel and then transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA). .. After blocking, the membranes were separately incubated with anti-SREBP1 antibody, anti-SREBP2 antibody, anti-HMGCR antibody (Santa Cruz Biotechnology, Dallas, TX, USA); anti-FASN antibody, anti-ACLY antibody (Abcam, Cambridge, UK); anti-cleaved caspase 3,anti-LC3IIB, anti-cytochrome C, anti-SCD1 antibody, anti-GAPDH antibody (Cell Signaling Technology, MA, USA) and anti-COX IV(Affinity, China) overnight at 4 °C, followed by incubation with the secondary antibodies for 1 h at room temperature. ..

    Blocking Assay:

    Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis
    Article Snippet: Subsequently, proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. After blocking with 5% bovine serum albumin (BSA) in TBST (Tris-buffered saline with Tween 20) for 1 h, the membranes were washed and incubated sequentially with a primary anti-SCD1 antibody (#2794, Cell Signaling Technology, Beverly, MA, USA) and anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies. .. Specific antibody binding was visualized using an enhanced chemiluminescence (ECL) detection reagent (Invitrogen, Carlsbad, CA, USA).

    Article Title: BF175 inhibits endometrial carcinoma through SREBP-regulated metabolic pathways in vitro
    Article Snippet: Then the proteins were separated on SDS-PAGE gel and then transferred to PVDF membranes (Millipore Corp., Billerica, MA, USA). .. After blocking, the membranes were separately incubated with anti-SREBP1 antibody, anti-SREBP2 antibody, anti-HMGCR antibody (Santa Cruz Biotechnology, Dallas, TX, USA); anti-FASN antibody, anti-ACLY antibody (Abcam, Cambridge, UK); anti-cleaved caspase 3,anti-LC3IIB, anti-cytochrome C, anti-SCD1 antibody, anti-GAPDH antibody (Cell Signaling Technology, MA, USA) and anti-COX IV(Affinity, China) overnight at 4 °C, followed by incubation with the secondary antibodies for 1 h at room temperature. ..

    Saline:

    Article Title: Therapeutic Potential of Stearoyl-CoA Desaturase1 (SCD1) in Modulating the Effects of Fatty Acids on Osteoporosis
    Article Snippet: Subsequently, proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). .. After blocking with 5% bovine serum albumin (BSA) in TBST (Tris-buffered saline with Tween 20) for 1 h, the membranes were washed and incubated sequentially with a primary anti-SCD1 antibody (#2794, Cell Signaling Technology, Beverly, MA, USA) and anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibodies. .. Specific antibody binding was visualized using an enhanced chemiluminescence (ECL) detection reagent (Invitrogen, Carlsbad, CA, USA).



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    Protein expression levels ( A , H ) including ( B ) PPARγ, ( C ) Fabp4, ( D ) CD36, ( E ) SREBP-1c, ( F ) ADIPOQ, ( G ) <t>SCD-1,</t> ( I ) TLR4, ( J ) IKKβ, and ( K ) p-P65 in liver tissues among NCD, HFCD, and HFCD + HB-1. Data were expressed as mean ± SD ( n = 3) based on Dunnett’s test. ** indicated p < 0.01, *** indicated p < 0.001, **** indicated p < 0.0001.
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    METTL1-deficient MSCs inhibit lipid synthesis in hepatocytes. (A) Representative images of Nile Red staining in hepatocytes co-cultured with MSC shGFP and MSC shMETTL1 following treatment with FFA (Scale bar = 20 μm). (B) Measurement of TG content in hepatocytes in the indicated groups. (C, D) Western blot analysis of lipid metabolism-related gene expression (FASN, SREBP1, <t>SCD1)</t> in the indicated groups. (E, F) qPCR analysis of lipid synthesis gene expression ( Fasn, Scd1, Srebp1, Fads1 and Acaca ) in AML12 and HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 . For all statistical graphs, data are presented as mean ± S.E.M, with statistical significance is indicated in the figure.
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    Image Search Results


    Protein expression levels ( A , H ) including ( B ) PPARγ, ( C ) Fabp4, ( D ) CD36, ( E ) SREBP-1c, ( F ) ADIPOQ, ( G ) SCD-1, ( I ) TLR4, ( J ) IKKβ, and ( K ) p-P65 in liver tissues among NCD, HFCD, and HFCD + HB-1. Data were expressed as mean ± SD ( n = 3) based on Dunnett’s test. ** indicated p < 0.01, *** indicated p < 0.001, **** indicated p < 0.0001.

    Journal: Foods

    Article Title: Chemical Composition Analysis of Highland Barley ( Hordeum vulgare L.) with Different Modification Methods and Lipid Metabolism Mechanism Analysis of Highland Barley with Microwave Fluidization Modification

    doi: 10.3390/foods15081396

    Figure Lengend Snippet: Protein expression levels ( A , H ) including ( B ) PPARγ, ( C ) Fabp4, ( D ) CD36, ( E ) SREBP-1c, ( F ) ADIPOQ, ( G ) SCD-1, ( I ) TLR4, ( J ) IKKβ, and ( K ) p-P65 in liver tissues among NCD, HFCD, and HFCD + HB-1. Data were expressed as mean ± SD ( n = 3) based on Dunnett’s test. ** indicated p < 0.01, *** indicated p < 0.001, **** indicated p < 0.0001.

    Article Snippet: The specific antibody concentrations used in this study were as follows: anti-rabbit antibodies against Peroxisome proliferator-activated receptor γ (PPARγ) (1:1000, 58 kDa, Affinity, 16643-1-AP), FABP4 (1:1000, 15 kDa, Affinity, DF6035), CD36 (1:1000, 88 kDa, Affinity, DF13262), SREBP-1c (1:1000, 122 kDa, Affinity, AF6283), ADIPOQ (1:1000, 26 kDa, Affinity, DF7000), SCD-1 (1:1000, 41 kDa, Bioss, bs-3787R), p-P65 (1:1000, 65 kDa, Affinity, AF2006, Serine Ser536), TLR4 (1:1000, 100 kDa, Affinity, AF7017), IKKβ (1:1000, 87 kDa, Affinity, AF6010), and GAPDH (1:1000, 37 kDa, Xianzhi Biotech, AB-P-R 001).

    Techniques: Expressing

    METTL1-deficient MSCs inhibit lipid synthesis in hepatocytes. (A) Representative images of Nile Red staining in hepatocytes co-cultured with MSC shGFP and MSC shMETTL1 following treatment with FFA (Scale bar = 20 μm). (B) Measurement of TG content in hepatocytes in the indicated groups. (C, D) Western blot analysis of lipid metabolism-related gene expression (FASN, SREBP1, SCD1) in the indicated groups. (E, F) qPCR analysis of lipid synthesis gene expression ( Fasn, Scd1, Srebp1, Fads1 and Acaca ) in AML12 and HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 . For all statistical graphs, data are presented as mean ± S.E.M, with statistical significance is indicated in the figure.

    Journal: Stem Cells Translational Medicine

    Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

    doi: 10.1093/stcltm/szag016

    Figure Lengend Snippet: METTL1-deficient MSCs inhibit lipid synthesis in hepatocytes. (A) Representative images of Nile Red staining in hepatocytes co-cultured with MSC shGFP and MSC shMETTL1 following treatment with FFA (Scale bar = 20 μm). (B) Measurement of TG content in hepatocytes in the indicated groups. (C, D) Western blot analysis of lipid metabolism-related gene expression (FASN, SREBP1, SCD1) in the indicated groups. (E, F) qPCR analysis of lipid synthesis gene expression ( Fasn, Scd1, Srebp1, Fads1 and Acaca ) in AML12 and HepG2 cells co-cultured with MSC shGFP and MSC shMETTL1 . For all statistical graphs, data are presented as mean ± S.E.M, with statistical significance is indicated in the figure.

    Article Snippet: Primary antibodies specific for the following proteins were obtained from Cell Signaling Technology: ACC-1 (#3676), SCD1 (#2794), FASN (#3180), p-AKT (#4060), and AKT (#9272).

    Techniques: Staining, Cell Culture, Western Blot, Gene Expression

    Transplantation of METTL1-deficient MSCs alleviates metabolic disorders associated with MASLD. (A) Schematic diagram of the animal experiment. (B) Evaluation of liver weight and the liver-to-body weight ratio in the indicated mice. (C) Assessment of fasting blood glucose levels in the indicated mice. (D) Analysis of GTT and ITT for the indicated groups. (E) Measurement of serum ALT and AST levels following 7 weeks of cell transplantation. (F) Representative images of HE and Oil Red O staining for analysis of mouse liver tissue (Scale bar = 100 μm). (G) Determination of TG and TC levels in the liver tissue of the specified mice. (H) qPCR analysis of lipid synthesis-related genes, including Fasn, Scd1, Srebp1, Fads1 , and Acaca in the specified groups. (I) Western blot analysis of lipid metabolism-related proteins in the specified groups. For all statistical graphs, individual data points represent individual mice, and data are presented as mean ± S.E.M. Statistical significance is indicated as shown in the figure.

    Journal: Stem Cells Translational Medicine

    Article Title: METTL1-deficient mesenchymal stem cells protect against metabolic-associated fatty liver disease by increasing NAMPT secretion

    doi: 10.1093/stcltm/szag016

    Figure Lengend Snippet: Transplantation of METTL1-deficient MSCs alleviates metabolic disorders associated with MASLD. (A) Schematic diagram of the animal experiment. (B) Evaluation of liver weight and the liver-to-body weight ratio in the indicated mice. (C) Assessment of fasting blood glucose levels in the indicated mice. (D) Analysis of GTT and ITT for the indicated groups. (E) Measurement of serum ALT and AST levels following 7 weeks of cell transplantation. (F) Representative images of HE and Oil Red O staining for analysis of mouse liver tissue (Scale bar = 100 μm). (G) Determination of TG and TC levels in the liver tissue of the specified mice. (H) qPCR analysis of lipid synthesis-related genes, including Fasn, Scd1, Srebp1, Fads1 , and Acaca in the specified groups. (I) Western blot analysis of lipid metabolism-related proteins in the specified groups. For all statistical graphs, individual data points represent individual mice, and data are presented as mean ± S.E.M. Statistical significance is indicated as shown in the figure.

    Article Snippet: Primary antibodies specific for the following proteins were obtained from Cell Signaling Technology: ACC-1 (#3676), SCD1 (#2794), FASN (#3180), p-AKT (#4060), and AKT (#9272).

    Techniques: Transplantation Assay, Staining, Western Blot